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ICDO
International
Castlemans
Disease
Organization

Molecular analysis of clonality in Castleman's disease.

Blood. 1995 Aug 1;86(3):1131-8. Unique Identifier : AIDSLINE MED/95345464
Soulier J; Grollet L; Oksenhendler E; Miclea JM; Cacoub P; Baruchel A; Brice P; Clauvel JP; d'Agay MF; Raphael M; et al; Laboratoire d'Hematologie Moleculaire, Hopital de Jour; Hematologie-Oncologie, Paris, France.

Abstract: Castleman's disease (CD) is a rare atypical lymphoproliferative disorder that is morphologically and clinically heterogenous and is associated with a risk of developing malignant lymphoma. We report the clonality status of CD tissues in 34 patients, including 14 patients infected by the human immunodeficiency virus (HIV). Four patients presented a localized form and 30 presented a multicentric form. Two cases were associated with B-cell lymphoma, 3 cases with Hodgkin's disease, and 9 cases (8 HIV+) with Kaposi's sarcoma. Histologically, 8 cases were of the hyaline-vascular type and 26 were of the plasma cell or mixed types. The Ig and T-cell receptor (TCR) V(D)J rearrangements were analyzed using polymerase chain reaction and Southern blot. Clonal IgH rearrangements were detected in only 4 cases, ie, 2 associated with B-cell lymphoma, 1 with Hodgkin's disease, and 1 case without malignancy. A TCR gamma rearrangement of restricted junctional size was amplified in 1 HIV+ case. Finally, polyclonal VH-JH and V gamma-J gamma rearrangements were detected in the large majority of the cases, irrespective of pathologic subtypes, clinical forms, and HIV status. The lymphoid component in CD is therefore commonly reactive, and the rare occurrence of detectable monoclonal lymphoid contingents may be caused by secondary molecular events.

Keywords: Adult Aged B-Lymphocytes/*CYTOLOGY Child Clone Cells Female Genes, Immunoglobulin Giant Lymph Node Hyperplasia/*PATHOLOGY Human HIV Infections/COMPLICATIONS Immunoglobulin Variable Region/GENETICS Immunoglobulins, Heavy-Chain/GENETICS Lymph Nodes/PATHOLOGY Male Middle Age Receptors, Antigen, T-Cell, gamma-delta/GENETICS Support, Non-U.S. Gov't T-Lymphocytes/*CYTOLOGY JOURNAL ARTICLE

SOURCE: National Library of Medicine. NOTICE: This material may be protected by Copyright Law (Title 17, U.S.Code).

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